• Just like a chameleon utilizing its
    colored pigments and crystals to
    adapt to its surroundings,
    we proactively pioneer and adapt to
    the everchanging NGS markets with
    our cutting-edge technology to
    support our customer’s needs.

Immune Repertoire Profiling Service (TCR/BCR)

Immune Repetoire often represents an individual’s current immuno-logic status,
whether healthy, vaccinated, diseased or infected

Overview

Immune Repertoire often represents an individual’s current immuno-
logical status, whether healthy, vaccinated, diseased or infected.

Only the high-throughput NGS analysis could comprehensively profile an individual’s
immune repertoire.
Celemics presents Immune Repertoire Profiling Service, providing effective data
acquisition, integration, and interpretation for the customers.
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Features &
Benefits

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    Quantitative analysis of the library diversity
    NGS-based analysis of complex antibody library consisting of millions
    (106 ~ 1012) of sequences in a single experiment
    Analysis of immunoglobulin and T-cell receptor repertoire
    analysis of BCR/TCR for each clone
    Frequency analysis of individual antibody clones within the library,
    identifying major and minor clones
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    Tracking of clonal frequencies for each sample
    For antibody discovery, analysis of library diversity according to
    its panning degree enabling monitoring changes in clonal frequency
    Minimized omission of potentially significant antibody clones
    Analysis of immune repertoire characteristics from blood
    sample and monitoring of each clone
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    Various analysis options for immune system studies
    Perform the experiment with drastically reduced time and cost enabled
    by the advanced technology of MSSIC developed from Celemics
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    Support various types of BI data analysis
    Celemics’ proprietary BI analysis pipeline provides the various, visible,
    and easy to read results for customer’s antibody libraries.

Workflow

General workflow of Immune Repertoire Profiling service

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Celemics provides service for data acquisition, integration and analysis, and interpretation.

Example of analysis report

Discovery of candidate antibodies from actively immunized chickens

CDR3 ID CDR3 AA p_pre-
immune
p_immune p_1st_boost p_2nd_boost p_tissue p_BM_2nd
CDR3sample_1 GSRDSSASTI 2433 773 31 0 2 461
CDR3sample_2 GSYDSSYVGI 1756 1444 2269 895 1058 789
CDR3sample_3 GSIDSSYVGI 1019 402 876 938 541 346
CDR3sample_4 ANFDSSSGAGI 46 25 338 483 1707 345
CDR3sample_5 GGYDSSAGI 231 268 934 207 966 7770
CDR3sample_6 GSFDSSTYAGI 3678 1034 425 290 547 431
CDR3sample_7 GSRDSSASTI 2433 773 31 0 2 461
CDR3sample_8 GSRDSSYVGI 6427 6370 10151 5756 10089 2680
CDR3sample_9 GGYDGSTYVGI 279 211 2047 178 271 88
CDR3sample_10 GSRDSNYVGI 407 567 974 749 868 224
CDR3sample_11 GSSSGTGI 1563 2580 899 1999 114 24702
CDR3sample_12 GSYDSSAGI 1195 875 1342 743 746 288
CDR3sample_13 GSRDSTYVGI 461 795 1355 998 983 136
CDR3sample_14 GGYDSSTDAGI 1167 1129 1353 1617 892 405

Analysis Report

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    Antibody Clustering by sequence
    With the clustering analysis, customers can obtain the
    immunizing status of the sample.
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    Example of network analysis
    Customers can easily recognize the relationship among the antibody
    library with visible network analysis

Example of network analysis

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Provide information of physical antibody status to select the target of the drug candidate

Applications

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    Discovery of novel drug candidate

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    Researches for Immunization