Overview
Library Preparation Kits are highly optimized for efficient usage with Celemics panels
The Library Preparation Kit Standard and Enzymatic Preparation (EP) both include end-repair and A-tailing enzyme mix index primers (single or dual), adapters and buffer.Description
LIBRARY PREPARATION WORKFLOW FOR TARGET ENRICHMENT NGS
| DNA Fragmentation | ||
| Standard Fragmentation | EP Fragmentation | |
| Option 1. Sonication | Option 2. Fragmentase | Fragmentase |
| Bead Purification & Quantification | ||
| NGS Library Preparation | ||
| ER / A | ER / A | ER / A |
| Adapter Ligation (Single / Dual Index) | ||
| Bead Purification | ||
| Index PCR | ||
| Target Enrichment | ||
Celemics provides two methods for library preparation step, Standard Library
Preparation Kit and Enzymatic Preparation Kit (EP Kit).
The Standard Library Preparation Kit includes all reagents for End repair (ER),
A-tailing (A), and Adapter Ligation steps. For DNA fragmentation from Standard
Library Preparation Kit, customers can use ultra-sonication devices or fragmentase.
Fragmentase is provided by Celemics and included in the kit uponrequest.
While Standard Kit is composed of 4 different steps, EP Kit includes all steps from
enzymatic fragmentation to ER/A in a single reaction enabling convenient workflow.
Since the purification step is not needed for EP Kit, the kit allows for minimal DNA
loss which is a crucial factor for damaged DNA samples such as FFPE.
- Note.
- For Option 1, ultra sonicator is not provided with the kit.
- For Option 2, the inclusion of the fragmentase in the kit is optional.
