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  Home > SERVICE > Next Generation Sequencing > Whole Exome Sequencing
Whole Exome Sequencing
Whole Exome Sequencing is determining the order of all the protein-coding genes in the genome and sequences the whole range of exons (exome) that contain information about protein expression profiles of human genes. SNV (Single Nucleotide Variant), InDel (Insertion/Deletion), CNV (Copy Number Variation), and rearrangement information can be obtained through Next Generation Sequencing applications.
※ Research Use Only. Not for human or animal therapeutic or diagnostic use.
  KEY FEATURES
• Detects structural variations, namely SNV, InDel, and CNV.
• Whole exome sequencing and analysis is available.
 
  APPLICATIONS
• Polygenic Disease Research
• Genetic Marker Development
 
  HOW TO PROCEED
Supply of the Gene list to Celemics
Quotation
(in 1 business day)
Send your samples
Experiment
Data Analysis
Payment
 
  SERVICE WORKFLOW
gDNA Fragmentation
Library Prep.
In-Solution
Hybridization
Post
Hybridization
Process
Amplication
NGS Run
Data Analysis
Data Report
 
  SAMPLE REQUIREMENT
Please send your inquiry for any samples which do not satisfy the minimum requirements described below. Samples should be prepared according to the guide below.
 
A. Human genomic DNA extracted from blood, tissue, saliva
 
Amount
Concentration
Purity
≥ 200 ng
≥ 10 ng/ul
A260/A280 = 1.8 - 2.0
※ Fluorometric quantitation method is employed to measure the amount of sample above (ex. Qubit, PicoGreen).
 
B. Human genomic DNA extracted from FFPE; Formalin-Fixed Paraffin-Embedded
Please send your inquiry for FFPE experiments to Celemics technical support team (support@celemics.com).
 
* Important Note
- For strongly degraded templates, such as FFPE samples, Celemics recommends to submit highest possible amount.
- And sequencing result may vary according to the quality of samples applied to a test.
 
* Additional Note
- Genomic DNA should be eluted in pure sterile water or TE buffer.
- Research use only
 
  TURNAROUND TIME
Domestic
4~6 Weeks
International
5~7 Weeks
※ For Whole Exome Sequencing, processing time is dependent upon the number of samples to be sequenced, as well as sequencing throughput.
 
  DELIVERABLES
Result
FASTQ / BAM-BAI / variant list / Summary stat
Delivery Method
FTP (<30GB), HDD (>30GB)
Shipping hard disk drive (HDD) can incur additional costs.
 
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