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  Home > SERVICE > Next Generation Sequencing > Targeted Sequencing
Targeted Sequencing
Targeted Sequencing obtains sequential DNA information through isolating specific subsets of genes or regions from the whole genome.
Celemics employs our own developed panels, which apply In-Solution Hybridization methodology. According to the unique and specific application needs of our clients, Celemics provides complete end to end service from customized panel design to Bioinformatics analysis.
※ Research Use Only. Not for human or animal therapeutic or diagnostic use.
  KEY FEATURES
• Produce a significant amount of data across Target regions at industry leading pricing
• High coverage & Uniformity
• A wide range of variant (SNV, InDel, CNV, and Gene Rearrangement) detection from small sample amounts
• Provides custom defined gene sets (~1.5Mb, Several hundreds of gene sets)
    ※ Please contact Celemics technical support team for target over 1.5 Mb.
 
  APPLICATIONS
• Cancer Research, Somatic mutation profiling
• Hereditary Disease
• Biological Research
 
  HOW TO PROCEED
Supply of the Gene list to Celemics
Quotation
(in 1 business day)
Probe Design
Design report confirmation by Customer
Send your samples
Sample Prep. and Sequencing
Data Analysis
Payment
* Optional Service after Design Report Confirmation
Pilot Test and Report Provision
Please contact Celemics technical support team if you want to request a pre-performance Pilot test before you order Targeted Sequencing services (support@celemics.com).
 
  SERVICE WORKFLOW
gDNA Fragmentation
Library Prep.
In-Solution
Hybridization
Post
Hybridization
Process
Amplication
NGS Run
Data Analysis
Data Report
 
  SAMPLE REQUIREMENT
Please send your inquiry for any samples which do not satisfy the minimum requirements described below. Samples should be prepared according to the guide below.
 
A. Human genomic DNA extracted from blood, tissue, saliva
 
Amount
Concentration
Purity
≥ 200 ng
≥ 10 ng/ul
A260/A280 = 1.8 - 2.0
※ Fluorometric quantitation method is employed to measure the amount of sample above (ex. Qubit, PicoGreen).
 
B. Human genomic DNA extracted from FFPE; Formalin-Fixed Paraffin-Embedded
Please send your inquiry for FFPE experiments to Celemics technical support team (support@celemics.com).
 
* Important Note
- For strongly degraded templates, such as FFPE samples, Celemics recommends to submit highest possible amount.
- And sequencing result may vary according to the quality of samples applied to a test.
 
* Additional Note
- Genomic DNA should be eluted in pure sterile water or TE buffer.
- Research use only
 
  TURNAROUND TIME
Domestic
4~6 Weeks
International
5~7 Weeks
 
  DELIVERABLES
Result
FASTQ / BAM-BAI / variant list / Summary stat
Delivery Method
FTP (<30GB), HDD (>30GB)
Shipping hard disk drive (HDD) can incur additional costs.
 
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