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  Home > SERVICE > Next Generation Sequencing > mRNA Sequencing
mRNA Sequencing
mRNA sequencing results are analyzed for the expression of genes, and also applicable for detecting SNV (Single Nucleotide Variant), InDel (Insertion/Deletion), and Gene Rearrangement mutations.
※ Research Use Only. Not for human or animal therapeutic or diagnostic use.
  KEY FEATURES
• Gene expression level detection
• Transcript Variants (isoforms) detection
• SNV(Single Nucleotide Variant), InDel(Insertion/Deletion), Gene Rearrangement detection
 
  HOW TO PROCEED
Supply of the Gene list to Celemics
Quotation
(in 1 business day)
Send your samples
Experiment
Data Analysis
Payment
 
  SERVICE WORKFLOW
mRNA Extraction
cDNA Synthesis
Library Prep.
NGS Run
Data Analysis
Data Report
 
  SAMPLE REQUIREMENT
Please send your inquiry for any samples which do not satisfy the minimum requirements described below. Samples should be prepared according to the guide below.
 
A. Extracted Human Total RNA
 
Amount
Concentration
Quality
≥ 500 ng
≥ 10 ng/ul
RNA integrity Number (RIN) ≥ 7
※ Fluorometric quantitation method is employed to measure the amount of sample above (ex. Qubit, PicoGreen).
 
B. Extracted Human Total RNA from FFPE tissue
Please send your inquiry for FFPE experiments to Celemics technical support team (support@celemics.com).
 
* Important Note
- For strongly degraded templates, such as FFPE samples, Celemics recommends to submit highest possible amount.
- And sequencing result may vary according to the quality of samples applied to a test.
 
* Additional Note
- RNA elution should be eluted in pure sterile water.
- Research use only
 
  TURNAROUND TIME
Domestic
4~6 Weeks
International
5~7 Weeks
 
  DELIVERABLES
Result
FASTQ / BAM-BAI / Analysis Report / Summary stat
Delivery Method
FTP (<30GB), HDD (>30GB)
Shipping hard disk drive (HDD) can incur additional costs.
 
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