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  Home > PRODUCT > Targeted Sequencing Kit > BRCA 1, 2 Kit
BRCA 1, 2 Kit
Celemics, on the basis of Target enrichment technology, has developed our own BRCA testing kit, which can detect mutations of the BRCA 1/BRCA 2 genes.
※ Research Use Only. Not for human or animal therapeutic or diagnostic use.
 
  GENERAL INFORMATION
Assay Genes
BRCA1 & BRCA2
Variants
SNV, InDel, CNV
Target Region
Promoter, UTR & CDS (+/- 30 bp, splicing site)
Applicable NGS platform
Illumina           HiSeq / NextSeq / MiSeq
Ion Torrent     PGM, Proton
 
  PRODUCT COMPOSITION
Standard Kit
Library Preparation Kit + Adaptors and Index Primers Set + BRCA 1,2 Target capture reagent (Probe, Hyb. Reagent and Wash Buffers)
All-in-one Kit
Standard Kit + TopQXSEP MagBead + Streptavidin Bead
 
  PRODUCT LIST
• All-in-one Kit for Illumina
Cat.no.
Quantity
 
AIBR16
BRCA 1, 2 kit (16rxn)
AIBR24
BRCA 1, 2 kit (24rxn)
AIBR48
BRCA 1, 2 kit (48rxn)
AIBR96
BRCA 1, 2 kit (96rxn)
 
• All-in-one Kit for Proton & PGM
Cat.no.
Quantity
 
APBR16
BRCA 1, 2 kit (16rxn)
APBR24
BRCA 1, 2 kit (24rxn)
APBR48
BRCA 1, 2 kit (48rxn)
APBR96
BRCA 1, 2 kit (96rxn)
 
• Standard Kit for Illumina
Cat.no.
Quantity
 
SIBR16
BRCA 1, 2 kit (16rxn)
SIBR24
BRCA 1, 2 kit (24rxn)
SIBR48
BRCA 1, 2 kit (48rxn)
SIBR96
BRCA 1, 2 kit (96rxn)
 
• Standard Kit for Proton & PGM
Cat.no.
Quantity
 
SPBR16
BRCA 1, 2 kit (16rxn)
SPBR24
BRCA 1, 2 kit (24rxn)
SPBR48
BRCA 1, 2 kit (48rxn)
SPBR96
BRCA 1, 2 kit (96rxn)
※ If you want some sample, please send an e-mail to Celemics technical support team (support@celemics.com).
 
  HOW TO PROCEED
Order
Quotation
(in 1 business day)
Payment
Shipment
 
  SAMPLE REQUIREMENT
Please send your inquiry for any samples which do not satisfy the minimum requirements described below. Samples should be prepared according to the guide below.
 
A. Human genomic DNA extracted from blood, tissue, saliva
 
Amount
Concentration
Purity
≥ 200 ng
≥ 10 ng/ul
A260/A280 = 1.8 - 2.0
※ Fluorometric quantitation method is employed to measure the amount of sample above (ex. Qubit, PicoGreen).
 
B. Human genomic DNA extracted from FFPE; Formalin-Fixed Paraffin-Embedded
Please send your inquiry for FFPE experiments to Celemics technical support team (support@celemics.com).
 
* Important Note
- For strongly degraded templates, such as FFPE samples, Celemics recommends to submit highest possible amount.
- And sequencing result may vary according to the quality of samples applied to a test.
 
* Additional Note
- Genomic DNA should be eluted in pure sterile water or TE buffer.
- Research use only
 
  REFERENCE
BRCA 1 and BRCA 2 are tumor suppressor genes and contribute to DNA repair of the cell, or the destruction of the cell if DNA cannot be repaired. Mutations of the BRCA 1 and BRCA 2 genes have been shown to contribute to the development of hereditary breast and ovarian cancers.

The chart below shows that mutations in the BRCA 1 and BRCA 2 genes can increase the risks of developing certain cancers.
 
- BRCA 1,2 - Associated Lifetime Risks by Cancer Type
 
1. Castera, L., et al., Next-generation sequencing for the diagnosis of hereditary breast and ovarian cancer using genomic capture targeting multiple candidate genes. <Eur J Hum Genet, 2014. 22(11): p. 1305-13>
2. Walsh, T., et al., Detection of inherited mutations for breast and ovarian cancer using genomic capture and massively parallel sequencing. <Proc Natl Acad Sci U S A, 2010. 107(28): p. 12629-33>
3. NCBI Gene Reviews. BRCA1 and BRCA2 Hereditary Breast and Ovarian Cancer
 
  TURNAROUND TIME
Domestic
2~3 Weeks
International
3~4 Weeks
 
  FOR YOUR BETTER EXPERIMENT....

TopQXSEP MagBead
All-in-one
Library Preparation Kit
 
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