CelemicsㅣInnovates DNA-Based Materials with novel sequencing technology

OVERVIEW

“ Celemics’ TrueRepertoire™ Solution overcomes the limitations of current antibody discovery methodologies and NGS-based immune repertoire analysis through ”: Because the in vitro screening method currently used in antibody discovery selects clones at random then
proceeds with an assay, there is a high chance of repeated discovery for antibodies existing in large quantities
and a low chance of discovery for antibodies of low frequencies with various characteristics and binding capacities.; In contrast, if the in vitro screening and DNA sequence analysis steps are switched to confirm the antibody
sequences inside the library and their binding capacities are checked without repeating identical antibodies,
clients are able to increase the number of clones with confirmed binding capacities and decrease the cost in
relation to the number of confirmed clones when compared to ELISA analysis.; Therefore, clients can discover rare clones previously undetected with existing technology and are also able to
drastically decrease the associated time and cost.

TrueRepertoire™

Celemics’ TrueRepertoire™ solution overcomes the limitations of current antibody discovery methodologies and NGS-based immune repertoire analysis

True Repertoire™ solution

1. Flawless analysis of an antibody library with over 10,000 sequences
2. Sequence analysis of the entire antibody variable area, including V_L-V_H Linkage information
3. Characteristic analysis and prediction of antibodies using specialized bioinformatics analysis
4. Retrieval of the client’s desired DNA antibody molecule

APPLICATIONS: Antibody discovery (Screening of candidate material); Lead antibody optimization; Single cell DNA sequence analysis

Provision of antibody DNA sequence library containing over 10,000 errorless strains: NGS-based sequence analysis and high-capacity
clone separation and molecular barcode assays
using Celemics’ proprietary MSSIC technology

V_L-V_H Linkage analysis of each antibody: Provision of V_L-V_H Linkage information, an area
previously difficult to analyze through
NGS due to its short read length

Provision of physical property analysis of each antibody through bioinformatics analysis: Clone frequency distribution within the library,
V_L and V_H sequence length distribution,
post-translation modification information,
CDR and frame amino acid information, etc.

Retrieval of selected physical antibody DNA from library: When using existing technologies to run NGS-based antibody sequence analysis, it is possible to obtain the sequence but impossible to get DNA material for each clone, requiring a costly gene synthesis process. Celemics’ TrueRepertoire Antibody Sequencing Service contains the client’s desired antibody clone within the library itself, eliminating the need to perform new gene fusions and decreasing the required time and cost.