Celemics | DNA-based Material Technology Company.

Meet Efficiency & Productivity

Celemics' Services

BTSeq™ Contiguous Sequencing Service

OVERVIEW

“ We were also able to significantly lower the Library Prep processing
and overall analysis costs by developing our own proprietary
BTSeq™ Library Preparation reagents, as these
factors occupied a significant portion of the cost of NGS analysis. ”
For the last few decades, Sanger Sequencing has been the standard for analyzing DNA sequences.
Due to its need for repetitive primer design, primer synthesis, and sequencing steps during Primer Walking
when analyzing long sequences, however, it requires lengthy experimental time and large costs to perform.
Additionally, issues such as high re-experimentation rates, intermittent errors, and a less than 1 kb read length
limitation have made sequence analysis difficult for clients.
To overcome these limitations, Celemics created an NGS-based molecular barcoding technology and NGS
error elimination algorithm solution, allowing for the analysis of sequences with lengths greater than 1kb
without the need of sequencing primers.

BTSeq™ Contiguous Sequencing Service

Using Celemics’ BTSeq™ Library Preparation reagents Contiguous Sequencing Service, clients are able to obtain accurate results, even from sequences that were difficult to read via Sanger Sequencing, such as those with strong secondary structures.

We were also able to significantly lower the Library Prep processing and overall analysis costs by developing our own proprietary BTPrep™ Library Preparation reagents, as these factors occupied a significant portion of the cost of NGS analysis.

APPLICATIONS
Long-range PCR amplicon sequencing
Plasmid sequencing
mtDNA sequence analysis
Microorganism identification
Miscellaneous Sanger sequencing applications
Efficient analysis without the
use of sequencing primers
Reduction in time and cost for primer production
and optimization (design, synthesis, testing)
originally required for Sanger Sequencing
Full length sequencing results for sequences
greater than 20 kb delivered within 2-3 days
Fast sequence analysis using Celemics’ proprietary
molecular barcode technology and
NGS error elimination/assembly algorithm
Implementation of NGS technology and
delivery of accurate and definite results
Use of digitized NGS analysis results to deviate
from Sanger sequencing results with obscure
peaks from excess noise
(AB1 and FASTA files included)
Very low re-experimentation rate
Provision of high-quality sequence information
without the need for re-experimentation
required by Sanger Sequencing
Accurate results generated from
strong secondary structures
Accurate results generated even from sequences
deemed difficult through Sanger Sequencing,
such as strong secondary structures
What to learn more about Celemics?
Send us a message and we will be happy to help you.